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1.  Nanoformulated ssRNA-based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer to Induce a Humoral Immune Response by Activation of Antigen-presenting Cells.LinkIT
Park HJ, Bang EK, Hong JJ, Lee SM, Ko HL, Kwak HW, Park H, Kang KW, Kim RH, Ryu SR, Kim G, Oh H, Kim HJ, Lee K, Kim M, Kim SY, Kim JO, El-Baz K, Lee H, Song M, Jeong DG, Keum G, Nam JH
Angewandte Chemie (International ed. in English), 2020
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0

2.  Double Crossed: A Case of La Crosse Encephalitis.LinkIT
Ding A, Shen B, Elliott S, Joshi K, Coghlin D, McLaughlin S
Rhode Island medical journal (2013), 2020
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0

3.  Isolation and Characterisation of Alongshan Virus in Russia.LinkIT
Kholodilov IS, Litov AG, Klimentov AS, Belova OA, Polienko AE, Nikitin NA, Shchetinin AM, Ivannikova AY, Bell-Sakyi L, Yakovlev AS, Bugmyrin SV, Bespyatova LA, Gmyl LV, Luchinina SV, Gmyl AP, Gushchin VA, Karganova GG
Viruses, 2020
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0

4.  Molecular Characterization of a Novel Strain of Fusarium graminearum Virus 1 Infecting Fusarium graminearum.LinkIT
Zhang L, Chen X, Bhattacharjee P, Shi Y, Guo L, Wang S
Viruses, 2020
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0

5.  Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA. S1097-2765(20)30109-X 10.1016/j.molcel.2020.02.016 Metazoan microRNAs require specific maturation steps initiated by Microprocessor, comprising Drosha and DGCR8. Lack of structural information for the assembled complex has hindered an understanding of how Microprocessor recognizes primary microRNA transcripts (pri-miRNAs). Here we present a cryoelectron microscopy structure of human Microprocessor with a pri-miRNA docked in the active site, poised for cleavage. The basal junction is recognized by a four-way intramolecular junction in Drosha, triggered by the Belt and Wedge regions that clamp over the ssRNA. The belt is important for efficiency and accuracy of pri-miRNA processing. Two dsRBDs form a molecular ruler to measure the stem length between the two dsRNA-ssRNA junctions. The specific organization of the dsRBDs near the apical junction is independent of Drosha core domains, as observed in a second structure in the partially docked state. Collectively, we derive a molecular model to explain how Microprocessor recognizes a pri-miRNA and accurately identifies the cleavage site. Copyright © 2020 Elsevier Inc. All rights reserved. Partin Alexander C AC Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Zhang Kaiming K Department of Bioengineering and James H. Clark Center, Stanford University, Stanford, CA 94305, USA. Jeong Byung-Cheon BC Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Herrell Emily E Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Li Shanshan S Department of Bioengineering and James H. Clark Center, Stanford University, Stanford, CA 94305, USA. Chiu Wah W Department of Bioengineering and James H. Clark Center, Stanford University, Stanford, CA 94305, USA; CryoEM and Bioimaging Division, SSRL, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA 94025, USA. Nam Yunsun Y Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: yunsun.nam@utsouthwestern.edu. eng Journal Article 2020 03 21 United States Mol Cell 9802571 1097-2765 IM DGCR8 Drosha Microprocessor RNA processing RNase III cryo-EM dsRBD microRNA pri-miRNA structure Declaration of Interests The authors declare no competing interests. 2019 12 18 2020 01 23 2020 02 19 2020 3 30 6 0 2020 3 30 6 0 2020 3 30 6 0 aheadofprint 32220646 S1097-2765(20)30109-X 10.1016/j.molcel.2020.02.016 32150360 NBK554776 StatPearls Publishing Treasure Island (FL) StatPearls 2020 01 2020 01 Internet Features, Evaluation and Treatment Coronavirus (COVID-19)LinkIT
Partin AC, Zhang K, Jeong BC, Herrell E, Li S, Chiu W, Nam Y, , Cascella M, Rajnik M, Cuomo A, Dulebohn SC, Di Napoli R
Molecular cell, 2020 Mar 21 Molecular cell Mol. Cell Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA. S1097-2765(20)30109-X 10.1016/j.molcel.2020.02.016 Metazoan microRNAs require specific maturation steps initiated by Microprocessor, comprising Drosha and DGCR8. Lack of structural information for the assembled complex has hindered an understanding of how Microprocessor recognizes primary microRNA transcripts (pri-miRNAs). Here we present a cryoelectron microscopy structure of human Microprocessor with a pri-miRNA docked in the active site, poised for cleavage. The basal junction is recognized by a four-way intramolecular junction in Drosha, triggered by the Belt and Wedge regions that clamp over the ssRNA. The belt is important for efficiency and accuracy of pri-miRNA processing. Two dsRBDs form a molecular ruler to measure the stem length between the two dsRNA-ssRNA junctions. The specific organization of the dsRBDs near the apical junction is independent of Drosha core domains, as observed in a second structure in the partially docked state. Collectively, we derive a molecular model to explain how Microprocessor recognizes a pri-miRNA and accurately identifies the cleavage site. Copyright © 2020 Elsevier Inc. All rights reserved. Partin Alexander C AC Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Zhang Kaiming K Department of Bioengineering and James H. Clark Center, Stanford University, Stanford, CA 94305, USA. Jeong Byung-Cheon BC Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Herrell Emily E Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Li Shanshan S Department of Bioengineering and James H. Clark Center, Stanford University, Stanford, CA 94305, USA. Chiu Wah W Department of Bioengineering and James H. Clark Center, Stanford University, Stanford, CA 94305, USA; CryoEM and Bioimaging Division, SSRL, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA 94025, USA. Nam Yunsun Y Laboratory of RNA Biology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Departments of Biophysics and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: yunsun.nam@utsouthwestern.edu. eng Journal Article 2020 03 21 United States Mol Cell 9802571 1097-2765 IM DGCR8 Drosha Microprocessor RNA processing RNase III cryo-EM dsRBD microRNA pri-miRNA structure Declaration of Interests The authors declare no competing interests. 2019 12 18 2020 01 23 2020 02 19 2020 3 30 6 0 2020 3 30 6 0 2020 3 30 6 0 aheadofprint 32220646 S1097-2765(20)30109-X 10.1016/j.molcel.2020.02.016 32150360 NBK554776 StatPearls Publishing Treasure Island (FL) StatPearls 2020
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0

6.  Atomic structure of potato virus X, the prototype of the Alphaflexiviridae family.LinkIT
Grinzato A, Kandiah E, Lico C, Betti C, Baschieri S, Zanotti G
Nature chemical biology, 2020
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0

7.  Evaluation of glycoprotein E subunit and live attenuated varicella-zoster virus vaccines formulated with a single-strand RNA-based adjuvant.LinkIT
Lee SJ, Park HJ, Ko HL, Lee JE, Lee HJ, Kim H, Nam JH
Immunity, inflammation and disease Immun Inflamm Dis Evaluation of glycoprotein E subunit and live attenuated varicella-zoster virus vaccines formulated with a single-strand RNA-based adjuvant. 10.1002/iid3.297 Varicella-zoster virus (VZV), a human alphaherpesvirus 3, elicits both chickenpox and shingles and/or postherpetic neuralgia. A live attenuated vaccine (LAV) and glycoprotein E (gE) subunit vaccine were developed to prevent VZV-induced diseases. We recently reported that single-strand RNA (ssRNA) based on the intergenic region of the internal ribosome entry site of cricket paralysis virus (CrPV) is an effective adjuvant for protein-based and virus-like particle-based vaccines. Here, Chinese hamster ovary expression system and an LAV from Oka/SK strains. We appraised the adjuvant effect of the same CrPV ssRNA encoding the gE gene formulated in the two vaccines using VZV-primed C57BL/6 mice and guinea pigs. Humoral immunity and cell-mediated immunity were assessed by enzyme-linked immunosorbent assay (ELISA) and ELISPOT in gE subunit vaccine and by ELISA and fluorescent antibody to membrane antigen in LAV. The gE subunit vaccine-induced gE-specific antibodies and CD4+ T-cell responses (indicated by interferon-? [IFN-?] and interleukin-2 secretion) in the ssRNA-based adjuvant containing the VZV gE gene. Therefore, an ssRNA adjuvant combined with gE antigen can trigger the innate immune response and induce an adaptive immune response to ultimately activate humoral and cell-mediated responses. VZV LAV could also induce VZV-specific antibodies and IFN-? stimulated by LAV, whereas the effect of ssRNA as a vaccine adjuvant could not be confirmed. However, the ssRNA adjuvant increased VZV-specific neutralizing antibody response. Taken together, these results highlight that the gE subunit vaccine and LAV developed in this study can be functional VZV vaccines, and ssRNAs appear to function better as adjuvants in a subunit vaccine than in an LAV. © 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. Lee Su Jeen SJ Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea. Department of R&D, SK Bioscience, Pangyoro, Bundang-gu, Republic of Korea. Park Hyo-Jung HJ Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea. Ko Hae Li HL Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea. Lee Jung Eun JE Department of R&D, SK Bioscience, Pangyoro, Bundang-gu, Republic of Korea. Lee Hyun Joo HJ Department of R&D, SK Bioscience, Pangyoro, Bundang-gu, Republic of Korea. Kim Hun H Department of R&D, SK Bioscience, Pangyoro, Bundang-gu, Republic of Korea. Nam Jae-Hwan JH http://orcid.org/0000-0002-0974-4343 Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea. eng HI15C2955 Ministry of Health & Welfare, Republic of Korea NRF-2015M3A9B5030157 Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning HD20A0323 The Korea Centers for Disease Control and Prevention The Catholic University of Korea, Research Fund, 2020 Journal Article 2020 03 13 England Immun Inflamm Dis 101635460 2050-4527 IM RNA adjuvant chickenpox gE subunit vaccine live attenuated vaccine shingles varicella-zoster virus 2019 12 21 2020 02 25 2020 02 27 2020 3 14 6 0 2020 3 14 6 0 2020 3 14 6 0 aheadofprint 32167678 10.1002/iid3.297 REFERENCES, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>8.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>The "Neglected Viruses" of <i>Taihu</i>: Abundant Transcripts for Viruses Infecting Eukaryotes and Their Potential Role in Phytoplankton Succession.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Pound HL, Gann ER, Tang X, Krausfeldt LE, Huff M, Staton ME, Talmy D, Wilhelm SW<br><font color=gray><i>Frontiers in microbiology, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>9.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Suppression of <i>Arabidopsis</i> Mediator Subunit-Encoding <i>MED18</i> Confers Broad Resistance Against DNA and RNA Viruses While <i>MED25</i> Is Required for Virus Defense.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Hussein NK, Sabr LJ, Lobo E, Booth J, Ariens E, Detchanamurthy S, Schenk PM<br><font color=gray><i>Frontiers in plant science, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br><br><br><table cellspacing=0 cellpadding=0 align=center><tr valign=bottom><td align=center><img src=p.png border=0></td><td align=center><img src=o_red.png border=0></td><td align=center><img src=rtal.png border=0></td></tr><td align=center></td><td align=center>1</td><td align=center></td></tr></table></table></tr></table></td><script src="http://www.google-analytics.com/urchin.js" type="text/javascript"> </script> <script type="text/javascript"> _uacct = "UA-634822-1"; urchinTracker(); </script> </BODY> </HTML>