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uBio  Web  Results 121 - 130 of about 3578 Synonyms:    Oncidium carthaginense (Coot Bay dancing-lady orchid)  Broader Terms:    Oncidium (Shower of gold)     Orchidales  More Specific:    Oncidium carthaginense andreanum     Oncidium carthaginense klotzschii     Oncidium carthaginense oerstedii     Oncidium carthaginense sanguineum     Oncidium carthaginense swartzii      External Resources: Common Names: Coot Bay dancing-lady orchid 121.  GeSUT4 mediates sucrose import at the symbiotic interface for carbon allocation of heterotrophic Gastrodia elata (Orchidaceae). Ho LH, Lee YI, Hsieh SY, Lin IS, Wu YC, Ko HY, Klemens PA, Neuhaus HE, Chen YM, Huang TP, Yeh CH, Guo WJ Plant, cell & environment Plant Cell Environ GeSUT4 mediates sucrose import at the symbiotic interface for carbon allocation of heterotrophic Gastrodia elata (Orchidaceae). 20-33 10.1111/pce.13833 Gastrodia elata, a fully mycoheterotrophic orchid without photosynthetic ability, only grows symbiotically with the fungus Armillaria. The mechanism of carbon distribution in this mycoheterotrophy is unknown. We detected high sucrose concentrations in all stages of Gastrodia tubers, suggesting sucrose may be the major sugar transported between fungus and orchid. Thick symplasm-isolated wall interfaces in colonized and adjacent large cells implied involvement of sucrose importers. Two sucrose transporter (SUT)-like genes, GeSUT4 and GeSUT3, were identified that were highly expressed in young Armillaria-colonized tubers. Yeast complementation and isotope tracer experiments confirmed that GeSUT4 functioned as a high-affinity sucrose-specific proton-dependent importer. Plasma-membrane/tonoplast localization of GeSUT4-GFP fusions and high RNA expression of GeSUT4 in symbiotic and large cells indicated that GeSUT4 likely functions in active sucrose transport for intercellular allocation and intracellular homeostasis. Transgenic Arabidopsis overexpressing GeSUT4 had larger leaves but were sensitive to excess sucrose and roots were colonized with fewer mutualistic Bacillus, supporting the role of GeSUT4 in regulating sugar allocation. This is not only the first documented carbon import system in a mycoheterotrophic interaction but also highlights the evolutionary importance of sucrose transporters for regulation of carbon flow in all types of plant-microbe interactions. © 2020 John Wiley & Sons Ltd. Ho Li-Hsuan LH Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan, Republic of China. Lee Yung-I YI Biology Department, National Museum of Natural Science, Taichung, Taiwan, Republic of China. Hsieh Shu-Ying SY Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan, Republic of China. Lin I-Shiuan IS Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan, Republic of China. Wu Yun-Chien YC Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan, Republic of China. Ko Han-Yu HY Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan, Republic of China. Klemens Patrick A PA Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany. Neuhaus H Ekkehard HE Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany. Chen Yi-Min YM Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan, Republic of China. Huang Tzu-Pi TP Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, Republic of China. Yeh Chih-Hsin CH Taoyuan District Agricultural Research and Extension Station, Council of Agriculture, Taoyuan, Taiwan, Republic of China. Guo Woei-Jiun WJ https://orcid.org/0000-0003-0183-8473 Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan, Republic of China. eng DAAD PPP Taiwan 57320939 Deutscher Akademischer Austauschdienst MOST 104-2311-B-006-004-MY3 Ministry of Science and Technology, Taiwan MOST 108-2314-B-006-77-MY3 Ministry of Science and Technology, Taiwan MOST PPP Germany 106-2911-I-006-506 Ministry of Science and Technology, Taiwan Journal Article 2020 08 10 United States Plant Cell Environ 9309004 0140-7791 IM Bacillus subtilis SUT mycoheterotrophy symbiosis transporter 2020 04 19 2020 06 17 2020 06 19 2020 6 26 6 0 2020 6 26 6 0 2020 6 26 6 0 ppublish 32583877 10.1111/pce.13833