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Broader Terms:
   Infectious 
   Megalocytivirus 

More Specific:
   African lampeye iridovirus 
   Dwarf gourami iridovirus 
   Giant seaperch iridovirus 
   Grouper sleepy disease iridovirus 
   Korean flounder iridovirus 
   Large yellow croaker iridovirus 
   Largemouth bass virus 
   Olive flounder iridovirus 
   Orange-spotted grouper iridovirus 
   Pearl gourami iridovirus 
   Red sea bream 
   Red sea bream iridovirus 
   Rock bream iridovirus 
   Sea bass 
   Sea bass iridovirus 
   Sea perch iridovirus 
   Silver gourami iridovirus 
   Turbot iridovirus 
   Turbot reddish body iridovirus 
 
 


External Resources:



1.  Simultaneous detection of multiple bacterial and viral aquatic pathogens using a fluorogenic loop-mediated isothermal amplification-based dual-sample microfluidic chip.LinkIT
Zhou QJ, Lu JF, Su XR, Jin JL, Li SY, Zhou Y, Wang L, Shao XB, Wang YH, Yan MC, Li MY, Chen J
Journal of fish diseases J Fish Dis Simultaneous detection of multiple bacterial and viral aquatic pathogens using a fluorogenic loop-mediated isothermal amplification-based dual-sample microfluidic chip. 401-413 10.1111/jfd.13325 Rapid and user-friendly diagnostic tests are necessary for early diagnosis and immediate detection of diseases, particularly for on-site screening of pathogenic microorganisms in aquaculture. In this study, we developed a dual-sample microfluidic chip integrated with a real-time fluorogenic loop-mediated isothermal amplification assay (dual-sample on-chip LAMP) to simultaneously detect 10 pathogenic microorganisms, that is Aeromonas hydrophila, Edwardsiella tarda, Vibrio harveyi, V. alginolyticus, V. anguillarum, V. parahaemolyticus, V. vulnificus, infectious hypodermal and haematopoietic necrosis virus, infectious spleen and kidney necrosis virus, and white spot syndrome virus. This on-chip LAMP provided a nearly automated protocol that can analyse two samples simultaneously, and the tests achieved limits of detection (LOD) ranging from 100 to 10-1  pg/?l for genomic DNA of tested bacteria and 10-4 to 10-5  pg/?l for recombinant plasmid DNA of tested viruses, with run times averaging less than 30 min. The coefficient of variation for the time-to-positive value was less than 10%, reflecting a robust reproducibility. The clinical sensitivity and specificity were 93.52% and 85.53%, respectively, compared to conventional microbiological or clinical methods. The on-chip LAMP assay provides an effective dual-sample and multiple pathogen analysis, and thus would be applicable to on-site detection and routine monitoring of multiple pathogens in aquaculture. © 2020 John Wiley & Sons Ltd. Zhou Qian-Jin QJ https://orcid.org/0000-0001-6363-6928 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China. School of Marine Sciences, Ningbo University, Ningbo, China. Lu Jian-Fei JF School of Marine Sciences, Ningbo University, Ningbo, China. Su Xiu-Rong XR State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China. School of Marine Sciences, Ningbo University, Ningbo, China. Jin Jing-Lei JL State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China. School of Marine Sciences, Ningbo University, Ningbo, China. Li Shang-Yang SY State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China. School of Marine Sciences, Ningbo University, Ningbo, China. Zhou Yan Y State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China. School of Marine Sciences, Ningbo University, Ningbo, China. Wang Lei L CapitalBio Corporation, Beijing, China. Shao Xin-Bin XB Zhejiang Mariculture Research Institute, Wenzhou, China. Wang Yao-Hua YH Zhejiang Mariculture Research Institute, Wenzhou, China. Yan Mao-Cang MC Zhejiang Mariculture Research Institute, Wenzhou, China. Li Ming-Yun MY School of Marine Sciences, Ningbo University, Ningbo, China. Chen Jiong J https://orcid.org/0000-0001-9389-520X State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China. School of Marine Sciences, Ningbo University, Ningbo, China. Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo, China. eng LGN18C180002 Program of Science and Technology Department of Zhejiang Province KF20200105 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products 2010DS700124-ZZ2008 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products 2015C110018 Scientific Innovation Team Project of Ningbo 2018A610342 Natural Science Foundation of Ningbo J2018004 Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource 2017B02 Jiangbei Agricultural and Social Development Science and Technology Project Journal Article 2020 12 19 England J Fish Dis 9881188 0140-7775 IM aquatic pathogen dual-sample microfluidic chip loop-mediated isothermal amplification multiplex detection real-time detection 2020 10 20 2020 12 02 2020 12 07 2020 12 20 6 0 2020 12 20 6 0 2020 12 19 12 5 ppublish 33340375 10.1111/jfd.13325 REFERENCES, 2021</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>2.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Refolded recombinant major capsid protein (MCP) from Infectious Spleen and Kidney Necrosis Virus (ISKNV) effectively stimulates serum specific antibody and immune related genes response in Nile tilapia (Oreochromis niloticus).</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Throngnumchai B, Jitrakorn S, Sangsuriya P, Unajak S, Khunrae P, Dong HT, Saksmerprome V, Rattanarojpong T<br><font color=gray><i>Protein expression and purification, 2021</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>3.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Two CD9 tetraspanin family members of Japanese flounder (Paralichthys olivaceus): characterization and comparative analysis of the anti-infectious immune function.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>He J, Gu H, Wang W, Hu Y<br><font color=gray><i>Veterinary research, 2021</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>4.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Transcriptional and subcellular characterization of interferon induced protein-35 (IFP35) in mandarin fish, Siniperca chuatsi.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Li L, Chen SN, Li N, Nie P<br><font color=gray><i>Developmental and comparative immunology, 2021</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>5.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Genomic and functional characterization of the lect2 gene from Siniperca chuatsi.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Shen Y, Cao M, Tang S, Zhao Y, Zhao J, Chen X, Bi Y<br><font color=gray><i>Fish & shellfish immunology, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>6.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Transcriptional programs of infectious spleen and kidney necrosis virus (ISKNV) in vitro and in vivo.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>He JH, Huang L, Guo Z, Weng S, He J, Xu X<br><font color=gray><i>Virus genes, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>7.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Identification of infectious spleen and kidney necrosis virus (ISKNV)-encoded microRNAs.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>He JH, Xia Q, Weng S, He J, Xu X<br><font color=gray><i>Virus genes, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>8.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Viral macrodomains: a structural and evolutionary assessment of the pharmacological potential.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Rack JGM, Zorzini V, Zhu Z, Schuller M, Ahel D, Ahel I<br><font color=gray><i>Open biology, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>9.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>Megalocytiviruses in ornamental fish: A review.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Johan CAC, Zainathan SC<br><font color=gray><i>Veterinary world, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br>10.  <a href=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0 class=title>First report of the infectious spleen and kidney necrosis virus (ISKNV) infection in ornamental fishes in India.</a><a href=http://ubio.org/tools/linkit.php?map%5B%5D=all&link_type=2&url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0><img src=linkit.png border=0 title='LinkIT' alt='LinkIT'></a> <br><span class=j>Girisha SK, Kushala KB, Nithin MS, Puneeth TG, Naveen Kumar BT, Vinay TN, Suresh T, Ajay SK, Venugopal MN, Ramesh KS<br><font color=gray><i>Transboundary and emerging diseases Transbound Emerg Dis First report of the infectious spleen and kidney necrosis virus (ISKNV) infection in ornamental fishes in India. 10.1111/tbed.13793 Infectious spleen and kidney necrosis virus (ISKNV), a member of family iridoviridae, reported for the first time in a wide range of ornamental fish species in India. Significant mortalities during the year 2018-19 were reported from a number of retailers in the region with various clinical signs. The samples of moribund, dead and apparently healthy ornamental fishes were collected from retailers, located in three districts of Karnataka, India. Out of 140 fish samples, 16 samples (11.42%) representing 10 different fish species were found positive to ISKNV by OIE listed primers and same samples were reported to amplify the major capsid protein (MCP) gene of ISKNV. Further, sequence analysis of MCP gene showed that all strains detected in this study were closely related to other documented isolates from different countries with an identity ranging from 98.76% to 100%. Further, they clustered in the clade of ISKNV, during the phylogenetic analysis. The sequence similarity was high (99.94%) to ISKNV strains from Japan, Australia and Malaysia. This is the first report of an ISKNV infection in India. Moreover, out of 10 ISKNV-positive fish species, three species were reported positive to ISKNV for the first time in the world. Further, the in vitro experiment showed the growth of virus in Asian sea bass cell line, which is a natural host of ISKNV. Therefore, considering the lethal nature of megalocytiviruses to infect a vast range of species, proper biosecurity measures need to be taken to control these emerging pathogens. © 2020 Wiley?VCH GmbH. Girisha S K SK https://orcid.org/0000-0003-0238-1954 College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. Kushala K B KB College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. Nithin M S MS College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. Puneeth T G TG College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. Naveen Kumar B T BT Department of Aquatic Environment, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India. Vinay T N TN Indian Council of Agricultural Research - Central Institute of Brackishwater Aquaculture, Chennai, India. Suresh T T College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. Ajay S K SK College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. Venugopal M N MN College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. Ramesh K S KS College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, India. eng National Fisheries Development Board and Department of Fisheries Journal Article 2020 08 16 Germany Transbound Emerg Dis 101319538 1865-1674 IM ISKNV MCP megalocytivirus ornamental fishes transboundary diseases 2020 04 20 2020 08 09 2020 08 10 2021 1 15 8 47 2021 1 16 6 0 2021 1 16 6 0 aheadofprint 33448668 10.1111/tbed.13793 REFERENCES, 2020</i></font><br><font color=#008000>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0<br></font></span><br><br><br><table cellspacing=0 cellpadding=0 align=center><tr valign=bottom><td align=center><img src=p.png border=0></td><td align=center><img src=o_red.png border=0></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=2><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=3><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=4><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=5><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=6><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=7><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=8><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=9><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=10><img src=o_yellow.png border=0></a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=2><img src=rtal.png border=0></a></td></tr><td align=center></td><td align=center>1</td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=2>2</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=3>3</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=4>4</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=5>5</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=6>6</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=7>7</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=8>8</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=9>9</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=10>10</a></td><td align=center><a href=http://ubio.org/portal/index.php?search=Infectious+spleen+kidney+necrosis+virus&category=l&client=pubmed&startPage=2>»</a></td></tr></table></table></tr></table></td><script src="http://www.google-analytics.com/urchin.js" type="text/javascript"> </script> <script type="text/javascript"> _uacct = "UA-634822-1"; urchinTracker(); </script> </BODY> </HTML>