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5. Response surface methodology optimised solvothermal system enables an efficient extraction of echinacoside and oleuropein from Syringa pubescens Turcz.
Wu Y, Chu Y, Yang Q, Li M, Yu M, Deng R, Liu K, Wang X, Fan E Phytochemical analysis : PCA Phytochem Anal Response surface methodology optimised solvothermal system enables an efficient extraction of echinacoside and oleuropein from Syringa pubescens Turcz. 10.1002/pca.3049 Syringa pubescens Turcz. was reported to be abundant in the Funiu Mountains of Henan Province and can be used to treat hepatitis and cirrhosis. In order to develop and utilise the resource, a fast and simple technique to extract bioactive compounds is needed. Our aims were to provide an extraction technique of glycosides from S. pubescens and study the antioxidant activity of this material. Box-Behnken design (BBD) was employed with three factors at three levels. The process parameters such as ethanol concentration (X1 ), temperature (X2 ), and solvent-solid ratio (X3 ) could significantly influence efficiency and yield of target compounds. High-performance liquid chromatography (HPLC) was used to determine the content of glycosides. DPPH (?,?-diphenyl-?-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and reducing power were used to evaluate the antioxidant activity of S. pubescens extract. The optimal parameters for the maximal extraction yield were obtained with ethanol concentration of 68%, temperature of 89°C, solvent-solid ratio of 46?mL/g, and time of 20?min. The S. pubescens extract showed strong antioxidant properties in vitro. The findings indicated the potential application of solvothermal extraction method to extract bioactive compounds from S. pubescens Turcz. Furthermore, the S. pubescens extract could be used as an important resource of antioxidant activity. © 2021 John Wiley & Sons, Ltd. Wu Yanfang Y School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang, China. Chu Yindi Y Department of Microbiology and Parasitology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing, China. State Key Laboratory of Medical Molecular Biology, Beijing, China. Yang Qinyu Q Chemical Engineering and Pharmaceutical School, Henan University of Science and Technology, Luoyang, China. Li Mengjia M School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang, China. Yu Miao M Chemical Engineering and Pharmaceutical School, Henan University of Science and Technology, Luoyang, China. Deng Ruixue R https://orcid.org/0000-0002-0301-0429 Chemical Engineering and Pharmaceutical School, Henan University of Science and Technology, Luoyang, China. Liu Kun K Chemical Engineering and Pharmaceutical School, Henan University of Science and Technology, Luoyang, China. Wang Xinsheng X https://orcid.org/0000-0002-4962-5023 Chemical Engineering and Pharmaceutical School, Henan University of Science and Technology, Luoyang, China. Fan Enguo E Department of Microbiology and Parasitology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing, China. State Key Laboratory of Medical Molecular Biology, Beijing, China. eng U1804175 National Natural Science Foundation of China No. U1804175 National Natural Science Foundation of China 2020144, 2020281 Student Research Training Programme Journal Article 2021 04 09 England Phytochem Anal 9200492 0958-0344 IM Funiu Mountain Syringa pubescens Turcz echinacoside and oleuropein response surface methodology solvothermal extraction 2021 03 15 2021 02 14 2021 03 22 2021 4 10 6 7 2021 4 11 6 0 2021 4 11 6 0 aheadofprint 33837595 10.1002/pca.3049 REFERENCES, 2021 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0
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